The principle of column chromatography is based on the ability of the constituents to
react with the solvent in the column
move at different speeds in the column
react with each other in the column
dissolve in each other in the column
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Explanation: Column chromatography works by separating components of a mixture based on their different affinities for the stationary phase (the adsorbent in the column) and the mobile phase (the solvent). Substances that interact more strongly with the stationary phase move more slowly, while those that interact more with the solvent move faster. Hence, separation occurs because the constituents move at different speeds in the column.
Final Answer: move at different speeds in the column
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